Fluorometer FLR200HS

Four E’s Scientific FLR200HS Laboratory Fluorometer

The Four E’s Scientific FLR200HS is a dedicated benchtop fluorometer engineered for high-sensitivity fluorescence-based quantification of nucleic acids and proteins in pharmaceutical, molecular biology, genomics, and clinical research laboratory environments. Designed for speed, sensitivity, and ease of use, the FLR200HS delivers accurate quantification results in just 2.5 seconds per sample — making it an efficient solution for laboratories processing large numbers of samples where reliable biomolecule quantification is a routine daily requirement.

With a fluorescence detection lower limit of 10pg/μL for dsDNA original sample concentration, the FLR200HS provides significantly greater sensitivity than standard UV-Vis absorbance measurement — making it particularly well suited to next-generation sequencing workflows, low-input library preparation, and any application where sample concentrations fall below the reliable detection range of conventional spectrophotometry.

Key Features

High Sensitivity Fluorescence Detection

The FLR200HS achieves a lower limit of detection of 10pg/μL for dsDNA — enabling accurate quantification of even minute quantities of biomolecules that would be undetectable by standard UV-Vis absorbance methods. The instrument supports a broad range of fluorescence quantitative analysis kits with pre-configured or custom standard curves, providing flexibility across diverse experimental requirements.

Fast Measurement — 2.5 Seconds Per Sample

Each fluorescence measurement is completed in just 2.5 seconds, enabling rapid processing of large sample batches without compromising accuracy. This throughput capability makes the FLR200HS well suited to high-throughput genomics workflows, NGS library preparation quality control, and other applications requiring fast, reliable quantification of multiple samples in sequence.

Broad Detection Range — 310–1000nm

The FLR200HS uses photodiode detectors covering a measurement range of 310–1000nm, providing broad spectral coverage for a wide range of fluorophores and quantification kits used in modern molecular biology and genomics applications.

Compact 0.5mL PCR Tube Sample Format

Samples are measured directly in standard 0.5mL PCR tubes using a sample volume of just 1–20μL — eliminating the need for specialised cuvettes or sample holders and simplifying the measurement workflow. This compact sample format is particularly practical in busy laboratory environments where minimising sample handling steps is important.

5.7-Inch Touchscreen Interface

The intuitive 5.7-inch touchscreen display provides a user-friendly interface for instrument operation, standard curve configuration, and result review — simplifying daily use and reducing the risk of operator error in routine quantification workflows.

Pre-Configured and Custom Standard Curves

The FLR200HS supports both pre-configured standard curves for commonly used fluorescence quantification kits and the ability to create custom standard curves for specialist applications — providing the flexibility required across diverse experimental workflows and kit formats.

Robust Data Storage and USB Export

The instrument stores up to 10,000 programs internally, providing extensive capacity for storing standard curves, protocols, and results. Data can be conveniently exported via USB drive for analysis, reporting, and integration with laboratory data management systems.

Technical Specifications

Applications

The FLR200HS Fluorometer is suited to a wide range of laboratory applications requiring high-sensitivity fluorescence-based quantification:

• DNA quantification — accurate dsDNA concentration measurement for molecular biology, cloning, and downstream analytical workflows
• RNA quantification — sensitive RNA concentration determination for gene expression analysis and transcriptomics applications
• NGS library preparation QC — fluorescence-based quality control of next-generation sequencing libraries prior to sequencing
• Protein quantification — fluorescence-based protein concentration determination using appropriate quantification kits
• Low-input genomics workflows — quantification of samples below the reliable detection range of standard UV-Vis spectrophotometry
• Pharmaceutical research and development — biomolecule quantification in drug discovery and formulation research workflows

FLR200HS vs SPT-NanoF — Choosing the Right Instrument

The FLR200HS is a dedicated fluorometer optimised specifically for fluorescence-based quantification, offering a compact and cost-effective solution for laboratories whose primary quantification requirement is fluorescence detection. For laboratories that additionally require full-spectrum UV-Vis spectrophotometry (190–800nm) and OD600 bacterial culture monitoring alongside fluorescence detection, the SPT-NanoF Spectrophotometer with Fluorescence Detection provides all three modalities in a single instrument. The SPT-Nano Microvolume Spectrophotometer is the appropriate choice for laboratories requiring UV-Vis spectrophotometry and OD600 measurement without fluorescence detection capability.

Availability in Ireland

The Four E’s Scientific FLR200HS Fluorometer is available from Varen Scientific — Ireland’s authorised distributor for Four E’s Scientific laboratory instruments. We provide local procurement support, clear lead-time communication, and documentation assistance for Irish pharmaceutical, research, and clinical laboratories sourcing laboratory instruments.

For further technical information on fluorescence-based nucleic acid quantification principles and method selection, refer to guidance published by the National Center for Biotechnology Information (NCBI).

To request a quote or discuss your fluorometry requirements, contact our team directly or use the Request a Quote button above.