Four E’s Scientific Optima 096 — 96-Well Gradient PCR Thermocycler
The Four E’s Scientific Optima 096 (PR122) is a high-performance 96-well gradient PCR thermocycler designed for precise and reproducible nucleic acid amplification in pharmaceutical, research, molecular biology, and clinical diagnostic laboratory environments. Featuring six independent temperature blocks with imported TEC modules, the Optima 096 delivers exceptional temperature control and gradient optimisation capability — making it an efficient and reliable solution for both routine PCR amplification and method development workflows.
The six independent temperature blocks allow simultaneous gradient temperature optimisation across multiple temperature profiles, significantly reducing the time required for annealing temperature optimisation during PCR method development — a critical workflow in regulated laboratory environments where method validation timelines are commercially important.
Key Features
Six Independent Temperature Blocks
Six independent temperature blocks with imported TEC (Thermoelectric Cooling) modules provide precise temperature control for PCR optimisation. Each block can be set to a different temperature within the gradient range, enabling simultaneous optimisation across multiple conditions in a single run.
Excellent Gradient Temperature Accuracy
The Optima 096 achieves a gradient temperature accuracy of ±0.1°C — ensuring highly consistent and reproducible amplification conditions across all 96 wells. This level of precision is particularly important in pharmaceutical and regulated laboratory environments where method reproducibility must be demonstrated and documented.
96-Well Capacity
The Optima 096 accommodates standard 96-well plates, 12×PCR 8-strip formats, and 96×0.2mL individual well plates, providing broad compatibility with common sample formats used in molecular biology and diagnostic laboratories. The PCR reaction volume range of 10–100μL supports a wide variety of amplification protocols.
Wide Sample Temperature Range
The sample temperature range of 4°C–100°C covers all standard PCR protocols including denaturation, annealing, and extension steps, as well as hold temperatures for short-term sample storage prior to downstream processing.
7-Inch LCD Touchscreen
The large 7-inch LCD touchscreen provides real-time display of the PCR process and can be operated comfortably while wearing laboratory gloves — an important practical consideration in busy molecular biology and pharmaceutical laboratory environments.
USB Interface for Program Management
The front-panel USB interface enables easy import and export of PCR programs and supports device software updates, simplifying protocol management and instrument maintenance across multiple users and applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Model | Optima 096 |
| Part Number | PR122 |
| Well Format | 96-well plates, 12×PCR 8-strip, 96×0.2mL well plates |
| PCR Reaction Volume | 10–100μL |
| Sample Temperature Range | 4°C–100°C |
| Temperature Blocks | Six independent blocks with TEC modules |
| Gradient Temperature Accuracy | ±0.1°C |
| Display | 7-inch LCD touchscreen |
| Connectivity | USB interface (front panel) |
Applications
- Routine PCR amplification — reliable and reproducible nucleic acid amplification for genotyping, cloning, and diagnostic testing
- Annealing temperature optimisation — simultaneous gradient optimisation across six temperature conditions in a single run
- PCR method development — efficient protocol development for new targets and primer combinations
- Pharmaceutical molecular biology — nucleic acid amplification in regulated pharmaceutical research and QC environments
- Clinical diagnostics — PCR-based diagnostic testing in clinical laboratory settings
Availability in Ireland
The Four E’s Scientific Optima 096 96-Well Gradient PCR Thermocycler is available from Varen Scientific — Ireland’s authorised distributor for Four E’s Scientific laboratory instruments. We provide local procurement support, clear lead-time communication, and documentation assistance for Irish pharmaceutical, research, and clinical laboratories.
For further information on gradient PCR optimisation principles, refer to guidance available from Thermo Fisher Scientific’s PCR learning centre.
To request a quote or discuss your PCR instrument requirements, contact our team or use the Request a Quote button above.
