Four E’s Scientific Microvolume Spectrophotometer with Fluorescence Detection
The Four E’s Scientific SPT-NanoF is an advanced microvolume spectrophotometer that combines full-spectrum UV-Vis spectrophotometry with integrated fluorescence detection capability in a single, compact instrument. Designed for pharmaceutical, molecular biology, genomics, and clinical research laboratories, the SPT-NanoF provides three distinct working modules — microvolume UV-Vis measurement, fluorescence-based nucleic acid quantification, and OD600 bacterial culture monitoring — making it one of the most versatile benchtop spectrophotometers available for laboratory use in Ireland.
With a fluorescence detection lower limit of 0.5pg/μL for dsDNA, the SPT-NanoF is capable of accurate quantification of extremely low-concentration nucleic acid samples that fall below the reliable detection range of standard UV-Vis spectrophotometry — making it an essential instrument for next-generation sequencing library preparation, single-cell genomics, and other low-input molecular biology workflows.
Key Features
Three Working Modules in One Instrument
The SPT-NanoF integrates three measurement modalities in a single benchtop instrument — microvolume UV-Vis spectrophotometry, fluorescence detection via 0.5mL PCR tube, and OD600 bacterial culture monitoring via cuvette. This eliminates the need for multiple separate instruments, reducing both laboratory bench space requirements and capital expenditure.
Fluorescence Detection — 0.5pg/μL Sensitivity
The fluorescence detection module uses a 0.5mL PCR tube sample holder with excitation filters at 435–485nm and emission filters at 530–560nm. It achieves a lower limit of detection of 0.5pg/μL for dsDNA — significantly more sensitive than UV-Vis absorbance — with a linear dynamic range of R²≥0.995 and repeatability of ≤1.5%. When used with fluorescence quantitative analysis kits, the SPT-NanoF accurately quantifies DNA and protein concentration through specific fluorophore binding.
Full Spectral UV-Vis Range 190–800nm
The SPT-NanoF covers a full spectral range of 190–800nm using a 2048px CMOS detector, with wavelength accuracy of 1nm and spectral resolution of ≤3nm — enabling accurate quantitative analysis of nucleic acids, proteins, and other biomolecules across all standard UV-Vis spectrophotometry applications.
Microvolume Sample Capability — 0.5–2μL
Only 0.5–2μL of sample is required for accurate and reproducible UV-Vis measurement — eliminating dilution requirements for high-concentration samples and conserving precious biological material. The aluminium alloy and quartz fibre sample pedestal ensures consistent path length and reliable results across repeated measurements, with automatic detection on lowering the pedestal arm.
Xenon Flash Lamp — No Warm-Up Required
The Xenon flash lamp light source provides immediate instrument readiness with no warm-up time required, along with exceptional measurement stability and a long service life of up to 10 years — reducing both downtime and the operational costs associated with conventional lamp replacement.
Standalone 7-Inch Colour Touchscreen
The built-in 7-inch LCD colour touchscreen provides a clear and intuitive user interface that allows all three detection modules to be operated without connecting to a computer. The Android-based software platform provides a familiar and responsive environment suited to busy laboratory settings.
OD600 Bacterial Culture Monitoring
The OD600 function via cuvette enables monitoring of bacterial and microorganism culture concentration using an LED light source at 600±8nm — supporting microbiology and cell biology applications alongside UV-Vis spectrophotometry and fluorescence-based quantification.
Data Output — Built-in Printer and USB Export
Results from all three measurement modules can be printed immediately via the built-in printer or exported via USB 2.0 for data analysis, reporting, and laboratory information management system integration.
Technical Specifications
| Parameter | Specification |
|---|---|
| Model | SPT-NanoF |
| Part Number | SPT-NanoF |
| Working Modules | Microvolume UV-Vis, Fluorescence Detection, OD600 (Cuvette) |
| Wavelength Range | 190–800nm |
| Minimum Sample Volume | 0.5–2μL |
| Path Length | 0.05mm / 0.2mm (high concentration), 1mm (normal concentration) |
| Light Source | Xenon flash lamp |
| Detector Type | 2048px, CMOS |
| Wavelength Accuracy | 1nm |
| Spectral Resolution | ≤3nm |
| Absorbance Precision | 0.003Abs |
| Absorbance Accuracy | 1% (4.096A at 260nm) |
| Absorbance Range | 0.04–300A |
| Lower Limit of Detection (UV-Vis) | 2 ng/μL (dsDNA) / 0.06 mg/mL (BSA) |
| Maximum Concentration | 15,000 ng/μL (dsDNA) / 440 mg/mL (BSA) |
| Sample Pedestal Material | Aluminium alloy and Quartz fibre |
| Fluorescence Sample Holder | 0.5mL PCR Tube (with PCR Tube Holder for Cuvette Slot) |
| Excitation Filters | 435–485nm |
| Emission Filters | 530–560nm |
| Fluorescence Lower Limit of Detection | 0.5pg/μL (dsDNA) |
| Linear Dynamic Range | R²≥0.995 |
| Repeatability | ≤1.5% |
| OD600 Sample Holder | Cuvette (12.5×12.5×45mm; Z-Height: 8.5mm) |
| OD600 Light Source | LED, 600±8nm |
| OD600 Absorbance Range | 0–4A |
| Display | 7-inch colour LCD touchscreen |
| Software | Android |
| Data Output | USB 2.0, built-in printer |
| Power Supply | AC100V–240V, 50Hz/60Hz (power adapter) |
| Operating Power | 48W |
| Standby Power | 8W |
| Dimensions (W×D×H) | 318×220×205mm |
| Weight | 4.1kg |
Applications
The SPT-NanoF Spectrophotometer with Fluorescence Detection is suited to demanding laboratory applications including:
- Next-generation sequencing library preparation — fluorescence-based quantification of low-input DNA libraries where UV-Vis sensitivity is insufficient
- Single-cell genomics — accurate quantification of extremely low-concentration nucleic acid samples
- DNA and RNA quantification — UV-Vis A260 measurement for standard molecular biology workflows
- Protein quantification — A280 absorbance and fluorescence-based protein concentration determination
- Bacterial culture monitoring — OD600 cell density measurement for microbiology applications
- Pharmaceutical research and development — comprehensive biomolecule quantification across multiple detection modalities
- Clinical research — sensitive nucleic acid detection for diagnostic research workflows
SPT-NanoF vs SPT-Nano — Choosing the Right Model
The SPT-NanoF is the advanced model, adding fluorescence detection to the standard UV-Vis and OD600 modules of the SPT-Nano Microvolume Spectrophotometer. If your laboratory requires quantification of very low-concentration nucleic acid samples — particularly for NGS library preparation or samples below 2ng/μL — the SPT-NanoF fluorescence module provides the sensitivity required. For standard UV-Vis spectrophotometry and OD600 applications, the SPT-Nano offers the same core platform at a lower cost.
Availability in Ireland
The Four E’s Scientific SPT-NanoF Spectrophotometer with Fluorescence Detection is available from Varen Scientific — Ireland’s authorised distributor for Four E’s Scientific laboratory instruments. We provide local procurement support, clear lead-time communication, and documentation assistance for Irish pharmaceutical, research, and clinical laboratories.
For further technical background on fluorescence-based nucleic acid quantification, refer to guidance published by the National Center for Biotechnology Information (NCBI) on nucleic acid quantification methods.
To request a quote or discuss which spectrophotometer model suits your laboratory application, contact our team or use the Request a Quote button above.
