Four E’s Scientific Microvolume Spectrophotometer SPT-Nano
The Four E’s Scientific SPT-Nano is a high-performance microvolume spectrophotometer designed for accurate quantitative analysis of nucleic acids, proteins, and other biomolecules in pharmaceutical, research, and clinical laboratory environments. Compact, intuitive, and ready for immediate use, the SPT-Nano delivers reliable UV-Vis spectrophotometry results across a full spectral range of 190–800nm without the need for a connected computer or lamp warm-up time.
The SPT-Nano operates with just 0.5–2μL of sample — making it ideal for precious or limited samples such as purified nucleic acids and low-yield protein preparations. Its Xenon flash lamp light source ensures immediate readiness and exceptional long-term stability, with a service life of up to 10 years.
Key Features
Standalone 7-Inch Colour Touchscreen
The built-in 7-inch LCD colour touchscreen provides a clear and intuitive user interface that allows all detection functions to be performed without connecting to a computer. This simplifies laboratory workflows and reduces IT infrastructure requirements in busy analytical environments.
Microvolume Sample Capability
The SPT-Nano requires only 0.5–2μL of sample for accurate and reproducible measurement, eliminating the need for dilution of high-concentration samples and conserving precious sample material. The aluminium alloy and quartz fibre sample pedestal ensures consistent path length and reliable results across repeated measurements.
Full Spectral Range 190–800nm
The SPT-Nano covers a full spectral range of 190–800nm using a 2048px CMOS detector, enabling accurate quantitative analysis across a wide range of applications including DNA and RNA quantification, protein analysis at A280, and bacterial culture monitoring at OD600 via cuvette.
Xenon Flash Lamp — No Warm-Up Required
The Xenon flash lamp light source ensures the SPT-Nano is ready for immediate measurement as soon as it is powered on — no warm-up time is required. The lamp provides exceptional stability and a long service life of up to 10 years, reducing consumable costs and instrument downtime.
Automatic Pedestal Arm Detection
The SPT-Nano features automatic sample detection on lowering the pedestal arm, streamlining the measurement process and reducing the risk of operator error during high-throughput sample quantification workflows.
OD600 Bacterial Culture Monitoring
The OD600 function via cuvette (12.5×12.5×45mm; Z-Height: 8.5mm) enables monitoring of bacterial and microorganism culture concentration using an LED light source at 600±8nm, supporting microbiology and cell biology applications alongside standard spectrophotometric measurements.
Data Output — Built-in Printer and USB Export
Results can be printed immediately via the built-in printer or exported via USB 2.0 for data analysis, archiving, and integration with laboratory information management systems. The Android-based software platform provides a familiar and responsive operating environment.
Technical Specifications
| Parameter | Specification |
|---|---|
| Model | SPT-Nano |
| Part Number | SPT-Nano |
| Wavelength Range | 190–800nm |
| Minimum Sample Volume | 0.5–2μL |
| Path Length | 0.05mm (high concentration) / 0.2mm (high concentration) / 1mm (normal concentration) |
| Light Source | Xenon flash lamp |
| Detector Type | 2048px, CMOS |
| Wavelength Accuracy | 1nm |
| Spectral Resolution | ≤3nm |
| Absorbance Precision | 0.003Abs |
| Absorbance Accuracy | 1% (4.096A at 260nm) |
| Absorbance Range | 0.04–300A |
| Lower Limit of Detection | 2 ng/μL (dsDNA) / 0.06 mg/mL (BSA) |
| Maximum Concentration | 15,000 ng/μL (dsDNA) / 440 mg/mL (BSA) |
| Sample Pedestal Material | Aluminium alloy and Quartz fibre |
| OD600 Sample Holder | Cuvette (12.5×12.5×45mm; Z-Height: 8.5mm) |
| OD600 Light Source | LED, 600±8nm |
| OD600 Absorbance Range | 0–4A |
| Display | 7-inch colour LCD touchscreen |
| Software | Android |
| Data Output | USB 2.0, built-in printer |
| Power Supply | AC100V–240V, 50Hz/60Hz (power adapter) |
| Operating Power | 48W |
| Standby Power | 8W |
| Dimensions (W×D×H) | 318×220×205mm |
| Weight | 4.1kg |
Applications
The SPT-Nano Microvolume Spectrophotometer is suited to a broad range of laboratory applications including:
- DNA and RNA quantification — accurate A260 measurement for molecular biology, genomics, and next-generation sequencing sample preparation workflows
- Protein quantification — A280 absorbance measurement for purified protein preparations in research and pharmaceutical development
- Bacterial culture monitoring — OD600 cell density measurement for microbiology and cell biology applications
- General UV-Vis spectrophotometry — full spectral scanning for analytical chemistry, pharmaceutical QC, and research applications
- Food industry analysis — detection of chemical substances and biomolecules in food quality testing environments
SPT-Nano vs SPT-NanoF — Which Model is Right for You?
The SPT-Nano provides two working modules: microvolume measurement and OD600 via cuvette — ideal for laboratories requiring standard UV-Vis spectrophotometry and bacterial culture monitoring. For laboratories that additionally require fluorescence-based quantification of extremely low concentration nucleic acids (lower limit 0.5pg/μL dsDNA), the SPT-NanoF Spectrophotometer with Fluorescence Detection provides a third working module using a 0.5mL PCR tube sample holder with excitation (435–485nm) and emission (530–560nm) filters.
Availability in Ireland
The Four E’s Scientific SPT-Nano Microvolume Spectrophotometer is available from Varen Scientific — Ireland’s authorised distributor for Four E’s Scientific laboratory instruments. We provide local procurement support, clear lead-time communication, and documentation assistance for Irish pharmaceutical, research, and clinical laboratories.
For further technical information on UV-Vis spectrophotometry and microvolume measurement principles, refer to guidance published by the National Center for Biotechnology Information (NCBI) on nucleic acid quantification methods.
To request a quote or discuss your spectrophotometry requirements, contact our team directly or use the Request a Quote button above.
